Analytical Services | Sequencing

DNA/RNA

Nanopore sequencing is a powerful and innovative technique that offers several advantages. Here are some reasons why researchers choose nanopore sequencing:

Long Read Lengths

Nanopore sequencing technology is capable of generating long read lengths compared to some other sequencing methods. Long reads are valuable for applications such as de novo genome assembly, haplotype phasing, and structural variant detection, as they can span repetitive regions and provide more comprehensive genomic information.

Single-Molecule Sequencing

Nanopore sequencing is a single-molecule sequencing method, meaning that it reads individual DNA molecules without the need for amplification. This can reduce biases introduced by amplification processes and provide a more accurate representation of the original DNA sample.

Direct RNA Sequencing

In addition to DNA sequencing, nanopore technology allows for direct sequencing of RNA molecules. This is particularly useful for studying RNA modifications, alternative splicing, and other aspects of RNA biology without the need for reverse transcription.

Minimal Sample Preparation

Nanopore sequencing requires relatively simple sample preparation compared to some other sequencing methods. This can save time and resources, making it a more accessible option for certain applications.

Detection of Modified Bases

Nanopore sequencing has the potential to detect modified bases in DNA, such as methylated cytosines. This capability is important for studying epigenetic modifications and understanding their roles in gene regulation and disease.

Rapid Turnaround Time

The real-time nature of nanopore sequencing allows for rapid generation of data. This can be advantageous in situations where quick results are essential, such as in clinical diagnostics or outbreak monitoring.

Continuous Improvements

Nanopore sequencing technology is actively evolving, with ongoing improvements in accuracy, throughput, and other performance metrics. This continuous development enhances the capabilities and applications of nanopore sequencing.